Plasmid

Part:BBa_K128008:Design

Designed by: Derek Ju   Group: iGEM08_MIT   (2008-10-29)


pJK650 plasmid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 7384
    Illegal XbaI site found at 7337
    Illegal XbaI site found at 7388
    Illegal SpeI site found at 944
    Illegal PstI site found at 7348
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 7384
    Illegal NheI site found at 1541
    Illegal SpeI site found at 944
    Illegal PstI site found at 7348
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 7384
    Illegal BglII site found at 1554
    Illegal BglII site found at 3036
    Illegal BglII site found at 5286
    Illegal BglII site found at 5442
    Illegal BglII site found at 6134
    Illegal BamHI site found at 7353
    Illegal XhoI site found at 155
    Illegal XhoI site found at 1502
    Illegal XhoI site found at 2621
    Illegal XhoI site found at 2714
    Illegal XhoI site found at 4370
    Illegal XhoI site found at 4769
    Illegal XhoI site found at 5043
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 7384
    Illegal XbaI site found at 7337
    Illegal XbaI site found at 7388
    Illegal SpeI site found at 944
    Illegal PstI site found at 7348
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 7384
    Illegal XbaI site found at 7337
    Illegal XbaI site found at 7388
    Illegal SpeI site found at 944
    Illegal PstI site found at 7348
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5956
    Illegal BsaI.rc site found at 2181
    Illegal BsaI.rc site found at 2866
    Illegal BsaI.rc site found at 6796
    Illegal SapI.rc site found at 4912


Design Notes

Compatible with only a few strains of Lactobacillus. Keep this in mind when attempting to use pJK650, since Lactobacillus can be picky with its range of plasmids



Source

Donated by Pascale Serror, IRNA, in France

References